Thanks to Max for noticing a pantsless Alfred on stilts looking worried on issue #103.
Alfred actually admits he's worried on the back cover of issue #210.
Looking about as pale as Michael Jackson on issue #438.
Thanks to R. Slick for pointing out issue #328.
As the water rises to his eyeballs, Alfred looks a little worried on issue #461.
Thanks to Dalton for noticing.
For issue #485, you be the judge.
Was he scared of the devils or worried for the country?
Probably a bit of both on issue #517.
Thanks to Mad fan Mark Forer for pointing out a couple of other more obscure examples:
Issue #48, page 33: "Future Confession Books":
Alfred has authored
"'I Worry!' America's most beloved idiot
wonders how long his success will last."
In Issue #47, page 18, in Dave Berg's xlnt article "Little League",
an ace kid pitcher "prospect"
is shown knockin' over a bunch of bottles in a carnival booth...
caption is: "Baseball scouts will search the country for talented kids."
The kid's wearin' an "I Worry" Alfred E. Neuman T-Shirt!
A.E.N. stamp turned into an unhappy expression).